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Making and Using Antibodies

A Practical Handbook

Edited by Gary C. Howard, Matthew R. Kaser

Contributors: Jory R. Baldridge, Kristi R. Harkins, Marit Nilsen-Hamilton, Frederic A. Fellouse, Paul Algate, Jose A. Ramos-Vara, David A. Fox, Lon V. Kendall, Kathleen Sheehan, Joseph Chandler, John Chen, Sara E. Miller, George P. Smith, Lee Bendickson, Elizabeth M. Smith, Sachdev S. Sidhu, Julie Ackerman Saettele, Sally P. Mossman, David N. Howell, M. Elaine Kunze

Published December 13th 2006 by CRC Press – 408 pages

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Description

Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.

Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-

  • Commercially available adjuvants designed for the production of antisera in the research setting

  • Methods for the production, purification, and characterization of antibodies

  • Practical guidance to researchers needing to modify antibodies

  • Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry

  • Methods for applying immunolabeling to the realm of electron microscopy (EM)

  • New antibody sources and new ways to use antobodies

    Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.

    • Contents

    Antibodies; Matthew R. Kaser and Gary C. Howard

    Antigens; Paul Algate, Jory Baldridge, and Sally Mossman

    Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman

    Production of Polyclonal Antibodies; Lon V. Kendall

    Production of Monoclonal Antibodies; Kathleen C. F. Sheehan

    Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith

    Purification and Characterization of Antibodies; Joseph P.

    Chandler

    Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu

    Chemical and Proteolytic Modification of Antibodies; George P. Smith

    Applications; Lee Bendickson and Marit Nilsen-Hamilton

    Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele

    Immunoelectron Microscopy; Sara E. Miller and David N. Howell

    Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze

    ELISAs; John Chen and Gary C. Howard

    Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard

    Name: Making and Using Antibodies: A Practical Handbook (Paperback) – CRC Press 
    Description: Edited by Gary C. Howard, Matthew R. KaserContributors: Jory R. Baldridge, Kristi R. Harkins, Marit Nilsen-Hamilton, Frederic A. Fellouse, Paul Algate, Jose A. Ramos-Vara, David A. Fox, Lon V. Kendall, Kathleen Sheehan, Joseph Chandler, John Chen, Sara E. Miller, George P. Smith, Lee Bendickson, Elizabeth M. Smith, Sachdev S. Sidhu, Julie Ackerman Saettele, Sally P. Mossman, David N. Howell, M. Elaine Kunze. Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to...
    Categories: Allergology & Clinical Immunology, Biochemistry, Cell Biology