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Laboratory Mouse and Laboratory Rat Procedural Techniques

Manuals and DVDs

By John J. Bogdanske, Scott Hubbard-Van Stelle, Margaret Rankin Riley, Beth M. Schiffman

CRC Press – 2010 – 192 pages

Purchasing Options:

  • Add to CartPaperback: $108.00
    978-1-43-985050-3
    November 21st 2010

Description

Despite the fact that the majority of research animals are rodents, the trainers at the Research Animal Resources Center at the University of Wisconsin-Madison found training material on the proper handling of mice and rats in biomedical research to be limited. So, they developed videos, narratives, pictures, and text to teach common handling, injection, and bleeding techniques.

The resulting DVDs and supporting manuals are complete training resources and refreshers for lab animal veterinarians, veterinary technicians, animal care staff, trainers, and researchers working with mice and rats.

Each DVD Provides:

  • Narrated video clips that demonstrate and describe each procedural technique
  • Easy navigation to desired techniques, including handling/transfer, oral gavage, blood draw, restraint, injection, and ear notching and tagging

Each Manual Includes:

  • Full text narratives and procedural handouts with full-color illustrations of each technique included on the DVD—including purpose and application of the procedure, recommended skills, and necessary supplies
  • Supplementary chapters that provide normative data for the laboratory rat and mouse

Buy the set and save! Two Manuals and Two DVDs

Also available as individual volumes:

Laboratory Mouse Procedural Techniques: Manual and DVD

Catalog no. K12294, November 2010, ISBN: 978-1-4398-5042-8

Laboratory Rat Procedural Techniques: Manual and DVD

Catalog no. K12295, November 2010, ISBN: 978-1-4398-5044-2

Reviews

Currently, there is a paucity of training material for experimental techniques in laboratory rodents, particularly audiovisual material. The manuals and accompanying DVDs will be of great interest to students, technicians, veterinarians, and investigators. Importantly, the straightforward approach taken in both the printed manual and DVD will be seen as an excellent tool for non-English speaking personnel.

-Mark A. Suckow, DVM, Dipl. ACLAM, Director, Freimann Life Science Center, University of Notre Dame, & Past President of AALAS

Persons (students of animal technology) can watch these videos to 'get the picture' and then practice with an experienced individual. It is a good refinement step of the 3Rs…. I will be very excited to have these manuals to use as training items with my students and as reference resources in our animal facility.

-Bruce W. Kennedy, MS, RLATG, CMAR, Compliance Associate, Research & Graduate Studies, California State Polytechnic University, Pomona, & Past President of AALAS

Contents

This Set Consists of Two Manuals and Two DVDs

Laboratory Mouse Procedural Techniques: Manual and DVD (Catalog no. K12294)

Section I: DVD and Voice-Over: Disease Management

Mouse Handling/Transfer

Mouse Restraint

Oral Gavage

One-Handed Injection Technique

Intraperitoneal (IP) Injection: One person

Intraperitoneal (IP) Injection: One-Person Towel Method

Subcutaneous (SQ) Injection

Subcutaneous (SQ) Injection: One-Person Towel Method

Maxillary Bleed

Pedal Vein Blood Draw

Saphenous Vein Blood Draw

Tail Vein Injection

Retro-Orbital Bleed: Anesthesia Required

Retro-Orbital Injections: Anesthesia Required

Ear Notching

Ear Tagging

Section II: Mouse Procedural Technique Handouts

Intraperitoneal (IP) Injection

Subcutaneous (SQ) Injection

Maxillary Bleed

Oral Gavage

Pedal Vein Blood Draw

Saphenous Vein Blood Draw

Retro-orbital Blood Draw

Retro-orbital Injection

Tail Vein Injection

Ear Notching/Punch & Ear Tags

The Mouse (Mus musculus) Class Outline: The Research Mouse

Normative Data

Research Animal Resources Center—Training Division: Normative Data for the Laboratory Mouse

Appendix: Blood Volume

Laboratory Rat Procedural Techniques: Manual and DVD (Catalog no. K12295)

Section I: DVD and Voice-Over: Disease Management

Rat Handling/Transfer

Rat Handling/Restraint

One-Handed Injection Technique

Intraperitoneal (IP) Injection: One-Person

Intraperitoneal (IP) Injection: Two-person

Intraperitoneal (IP) Injection: One-Person Towel Method

Subcutaneous (SQ) Injection: One-Person Towel Method

Pedal Vein Blood Draw

Saphenous Vein Blood Draw

Oral Gavage

Jugular Bleed (Two-Person)

Tail Artery Blood Draw

Tail Vein Injection

Ear Notching

Ear Tagging

Section II: Rat Procedural Technique Handouts

Intraperitoneal (IP) Injection

Subcutaneous (SQ) Injection: Towel Method

Jugular Bleed

Oral Gavage

Pedal Vein Blood Draw

Saphenous Vein Blood Draw

Tail Artery Blood Draw

Tail Vein Injection

Ear Notch/Punch & Ear Tags

The Rat (Rattus norvegicus): The Research Rat

Research Animal Resources Center—Training Division: Normative Data for the Laboratory Rat

Appendix: Blood Volume

Author Bio

John Bogdanske

After graduating from Augustana College, John started his research career in 1986 at the University of Wisconsin-Madison, Department of Nutritional Sciences working with dietary fiber and the nutritional value of common foods. In 1991 he moved to the School of Veterinary Medicine on the Madison campus to take the position of Lab Manager in the Comparative Orthopaedic Research Laboratory (CORL), Department of Medical Sciences. The CORL’s mission was to investigate fundamental orthopaedic problems that affected either animals or humans in order to identify the mechanisms that cause disease or to develop treatments that would enhance the lives of individuals affected with these diseases. In 2004 he joined the Research Animal Resources Center (RARC) as a Training Coordinator. Along with Scott, Margaret and Beth, he helps develop, manage, and present a comprehensive training program to all UW-Madison research animal users and related staff. John is an active member of Laboratory Animal Welfare Training Exchange (LAWTE) and the American Association for Laboratory Animal Science (AALAS). John spends most of his free time with his family usually playing in the outdoors (on a bike).

Scott Hubbard-Van Stelle

Scott attended the University of Wisconsin in the pre-veterinary program before getting his degree as a certified veterinary technician from the Madison Area Technical College. He worked in a veterinary hospital for a small animal practitioner as a surgical nurse, followed by working for Raltech (now Covance) as a sample collection specialist. Scott started his career at the University of Wisconsin in 1980 when he took a position as a Veterinary Technician in the Veterinary Science Department (currently the department of Animal Health and Biomedical Sciences). After several years, he was promoted to Sr. Research Specialist. During his 19 years with the department, Scott set up the teaching labs and assisted in the teaching of the 301 Physiology and 701 Experimental Surgical Techniques courses, was the assistant to the large animal Experiment Station veterinarian, and supervised the animal care facility for the department. Scott was certified as a Laboratory Animal Technologist in 1993 through the American Association for Laboratory Animal Science, and is an active member in the Laboratory Animal Welfare Training Exchange. In 2000 Scott accepted his current position as a Training Coordinator with the Research Animal Resources Center at the University of Wisconsin-Madison. Scott’s dogs, guitar, and outdoor activities fill his leisure hours.

Margaret Rankin Riley

After graduating in 1982 with a BS from the University of Wisconsin-Madison, Margaret began her UW Madison research work in 1983 at the School of Veterinary Medicine in the respiratory physiology laboratory. In 1990 she moved to the Department of Surgery at UW hospital and worked in the organ preservation research laboratory as a Research Specialist and then became lab manager of the Experimental Surgery Department. Margaret worked with many large and small animal models and assisted researchers with their surgical procedures. In 2004, Margaret joined the Research Animal Resources Center (RARC) as a Training Coordinator where she manages and presents a comprehensive training program to all UW research animal users and their staff. Margaret is an active member of the American Association for Laboratory Animal Science (AALAS) and the Laboratory Animal Welfare Training Exchange (LAWTE). Margaret spends most of her free time on the cross country ski, road and mountain bike trails throughout Wisconsin.

Beth Schiffman

Beth became a Certified Veterinary Technician in 1990 from Madison Area Technical College (Wisconsin). In 1991 she graduated as a Veterinary Technologist from Morehead State University (Kentucky) with a Bachelor of Science.

In 1989 Beth began working in a mixed-practice veterinary hospital in Mukwonago, Wisconsin, and she also spent time interning at the Milwaukee County Zoo. In 1993 she left private practice and began her research animal career at Hazleton Laboratories in Madison, Wisconsin. Pursuing an opportunity at the University of Wisconsin – Madison in 1996, Beth began work as a Research Specialist for the Medical School and later became interim manager for the Animal Care Unit. In 1999 she joined the University’s Research Animal Resources Center as a Training Coordinator where she develops, manages, and presents a comprehensive training program to all UW research animal users and their staff. Beth obtained her RLATG certification and is an active member of the American Association for Laboratory Animal Science and the Laboratory Animal Welfare Training Exchange. Most of her free time is spent with her family, horses, and dog or training and competing in running, biking, and triathlon events.

Name: Laboratory Mouse and Laboratory Rat Procedural Techniques: Manuals and DVDs (Paperback)CRC Press 
Description: By John J. Bogdanske, Scott Hubbard-Van Stelle, Margaret Rankin Riley, Beth M. Schiffman. Despite the fact that the majority of research animals are rodents, the trainers at the Research Animal Resources Center at the University of Wisconsin-Madison found training material on the proper handling of mice and rats in biomedical research to be...
Categories: Neuroscience, Toxicology, Laboratory Animal Science