Making and Using Antibodies
A Practical Handbook, Second Edition
Edited by Gary C. Howard, Matthew R. Kaser
To Be Published July 18th 2013 by CRC Press – 453 pages
This second edition of a popular text provides clear guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present clear descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included.
Introduction, Kaser and Howard
Antigens And Adjuvants, Algate
Polyclonal Antibodies, Kendall
Monoclonal Antibodies, Sheehan
Purification and Charaterization, Chandler
Bacteria and Phage, Sidhu
Quantitative Production of Monoclonal Antibodies, Fox and Smith
Modifying Antibodies, Smith
Immune-Electron Microscopy, Howell and Miller
Flow Cytometry, Harkins and McClellan
ELISAs, Chen and Howard
Humanized Antibodies, Almagro and Fransson
Antibodies in the Future, Howard and Kaser
Gary C Howard is Principal Scientific Editor at The David Gladstone Institutes in San Francisco, California.
Matthew R Kaser is Partner at Bell and Associates in San Francisco, California.