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Making and Using Antibodies

A Practical Handbook, Second Edition

Edited by Gary C. Howard, Matthew R. Kaser

To Be Published July 18th 2013 by CRC Press – 453 pages

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Description

This second edition of a popular text provides clear guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present clear descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included.

Contents

Introduction, Kaser and Howard

Antigens And Adjuvants, Algate

Polyclonal Antibodies, Kendall

Monoclonal Antibodies, Sheehan

Purification and Charaterization, Chandler

Bacteria and Phage, Sidhu

Quantitative Production of Monoclonal Antibodies, Fox and Smith

Aptamers, Nilsen-Hamilton

Modifying Antibodies, Smith

Westerns, Nilsen-Hamilton

Immunohistochemistry, Ramos-Vara

Immune-Electron Microscopy, Howell and Miller

Flow Cytometry, Harkins and McClellan

ELISAs, Chen and Howard

Humanized Antibodies, Almagro and Fransson

Antibodies in the Future, Howard and Kaser

　

　

Author Bio

Gary C Howard is Principal Scientific Editor at The David Gladstone Institutes in San Francisco, California.

Matthew R Kaser is Partner at Bell and Associates in San Francisco, California.

Name: Making and Using Antibodies: A Practical Handbook, Second Edition (Paperback) – CRC Press 
Description: Edited by Gary C. Howard, Matthew R. Kaser. This second edition of a popular text provides clear guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes...
Categories: Biochemistry, Immunology, Drug Design & Development