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Introduction to Instrumentation in Life Sciences

By Prakash Singh Bisen, Anjana Sharma

CRC Press – 2013 – 383 pages

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  • Add to CartPaperback: $83.95
    978-1-46-651240-5
    September 25th 2012

Description

Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the physical principles involved in the operation of research instruments and the parameters required in using them. Introduction to Instrumentation in Life Sciences fills this need by addressing different aspects of tools that hold the keys to cutting-edge research and innovative applications, from basic techniques to advanced instrumentation. The text describes all topics so even beginners can easily understand the theoretical and practical aspects.

Comprehensive chapters encompass well-defined methodology that describes the instruments and their corresponding applications in different scientific fields. The book covers optical and electron microscopy; micrometry, especially in microbial taxonomy; pH meters and oxygen electrodes; chromatography for separation and purification of products from complex mixtures; spectroscopic and spectrophotometric techniques to determine structure and function of biomolecules; preparative and analytical centrifugation; electrophoretic techniques; x-ray microanalysis including crystallography; applications of radioactivity, including autoradiography and radioimmunoassays; and fermentation technology and subsequent separation of products of interest.

The book is designed to serve a wide range of students and researchers in diversified fields of life sciences: pharmacy, biotechnology, microbiology, biochemistry, and environmental sciences. It introduces different aspects of basic experimental methods and instrumentation. The book is unique in its broad subject coverage, incorporating fundamental techniques as well as applications of modern molecular and proteomic tools that are the basis for state-of-the-art research. The text emphasizes techniques encountered both in practical classes and in high-throughput environments used in modern industry. As a further aid to students, the authors provide well-illustrated diagrams to explain the principles and theories behind the instruments described.

Reviews

"The proposed book is an excellent one for use of students, research scholars and teachers of life sciences, biotechnology, biochemistry, biophysics and other related disciplines where such books exclusively dedicated for life sciences are not available."

—Prof. Sukh Mahendra Singh, Coordinator, School of Biotechnology, Banaras Hindu University, India

"A comprehensive text book on instrumentation methods which covers all major topics including practical exercises representing the syllabi of various postgraduate and undergraduate programmes in life sciences."

—Prof. Vinay Sharma, Prof. & Head, Dept of Bioscience & Biotechnology, Banasthali University, India

"I find that the book should be much useful for undergraduate (bachelors degree) and graduate (masters degree) students. Very good book, properly arranged topics, will certainly be useful for the audience."

—Anil Kumar, Ph.D., Professor and Head (Chair), School of Biotechnology, Devi Ahilya University, India

Contents

Microscopy

Introduction

Magnification, Resolution, and Contrast

Light (Bright Field) Microscopy

Dark Field Microscope

Phase Contrast Microscope

Interference Microscope

UV and Fluorescence Microscopes

Electron Microscopy

Tunneling Electron Microscopy

Confocal Microscopy

Techniques in Microscopy

Electron Microscopy

Suggested Readings

Important Links

Micrometry

Introduction

Structure of an Ocular Micrometer

Conjugate Image-Forming Focal Planes

Eyepiece Designs

Stage Micrometer

Filar Eyepiece Micrometer

Principle of Filar Eyepiece Micrometer

Working

Suggested Reading

Important Links

Electrochemical Techniques

Introduction

Structure of a pH Meter

Principles

Factors Limiting the Accuracy of pH Measurements

Measurement of pH

Working

Procedure for Measuring pH Using a pH Meter

Cleaning and Troubleshooting of Glass Probes

Application of pH Measurements

Oxygen Electrode

Suggested Readings

Important Links

Chromatography

Introduction

General Principles

Column Chromatography

Paper Chromatography and Thin-Layer Chromatography

Sample Collection, Preservation, and Preparation

Adsorption Chromatography

Partition Chromatography

High-Performance Liquid Chromatography

Fast Protein Liquid Chromatography

Gas–Liquid Chromatography

Ion-Exchange Chromatography

Exclusion Chromatography

Affinity Chromatography

Suggested Readings

Important Links

Spectroscopy

Introduction

UV–Visible Spectroscopy

IR (Vibrational) Spectroscopy

Flame/Atomic Absorption Spectroscopy

Fluorescence Spectroscopy

ESR Spectroscopy

NMR Spectroscopy

MALDI-TOF Mass Spectrometry

Circular Dichroism (CD) Spectroscopy

Suggested Readings

Important Links

Centrifugation

Introduction

Principle of Centrifugation

Types of Centrifuges

Types of Centrifugal Separations

Rotor Categories

Selection of Centrifuge Tubes

Common Centrifugation Vocabulary and Formulas

Analytical Ultracentrifuge

Suggested Reading

Important Links

Electrophoresis

Introduction

Principles of GE

Working with the Electrophoresis Apparatus

Denaturing Gradient GE

Temperature Gradient GE

Pulsed-Field GE

Capillary Electrophoresis

Suggested Readings

Important Links

X-Ray Microanalysis

Introduction

Principles

Instrumentation

Applications of X-Ray Microanalysis

Techniques for the Analysis of Secondary, Tertiary, and Quaternary Proteins by X-Ray Crystallography

Suggested Readings

Important Link

Techniques with Radioisotopes

Introduction

Isotopes and Radioactivity

Ionization Effects

Measurement Units

Measurement Techniques

Autoradiography

Counting Statistics

Biological Uses of Radioisotopes

Tracer Dilution Technique

Radioimmunoassay

Miscellaneous Uses of Radioisotopes

Radioisotope Production

Most Widely Used Radioisotopes

Suggested Reading

Important Links

Fermentation

Introduction

History

Principles of Fermentation

Introduction to Microorganisms

Types of Fermentation Metabolism

Fermentation Process

Types of Fermentation

Types of Fermentors

Sterilization in Fermentation

Product Recovery

Suggested Readings

Important Links

Conductivity Meter

Introduction

Principles

Common Definitions

Conductivity Meter

Conductivity Cells

Factors Influencing the Measurement

Measuring Techniques

Reliable Measurements

Maintenance and Storage

Applications

Suggested Readings

Important Links

Glossary

Index

Name: Introduction to Instrumentation in Life Sciences (Paperback)CRC Press 
Description: By Prakash Singh Bisen, Anjana Sharma. Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the...
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